Device

Part:BBa_K2936019:Design

Designed by: Weng Jiaqi   Group: iGEM19_ZJUT-China   (2019-10-10)


pBAD-lacZ alpha-pFrmR-AraC-CAT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3178
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3178
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 120
    Illegal BamHI site found at 182
    Illegal BamHI site found at 2421
    Illegal XhoI site found at 3214
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3178
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3178
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a leak problem with the FrmR promoter and the colors have a overlap when the concentration of formaldehyde changes.


Source

Escherichia coli

References